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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Acetyl Coenzyme A Carboxylase ELISA kit (E11A0913)

E11A0913 Bovine Acetyl Coenzyme A Carboxylase ELISA kit

Bovine Acetyl Coenzyme A Carboxylase ELISA kit is suitable for the detection of samples from Bovine species. Acetyl Coenzyme A Carboxylase can also be called ACC.

Products

Specifications of Bovine Acetyl Coenzyme A Carboxylase ELISA kit

Product Information

Cat. NO.

E11A0913

Product Name

Bovine Acetyl Coenzyme A Carboxylase ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

A competitive enzyme immunoassay approach is used in the ACC ELISA kit along with an anti-ACC antibody and an ACC-HRP conjugate. For one hour, the ACC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the ACC concentration since ACC from samples and ACC-HRP conjugate compete for the anti-ACC antibody binding site. Since the number of sites is limited, as more sites are occupied by ACC from the sample, fewer sites are left to bind ACC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACC concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Acetyl Coenzyme A Carboxylase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-106%

Linearity

Diluent Ratio

Range %

1:2

89-109

1:4

88-112

1:8

85-111

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ACC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Acetyl Coenzyme A Carboxylase ELISA kit

Summary of the Assay Procedure for Bovine Acetyl Coenzyme A Carboxylase ELISA kit

Citations of Bovine Acetyl Coenzyme A Carboxylase ELISA Kit

E11A0913 has been referenced in the below publications:

Acetic Acid Activates the AMP-Activated Protein Kinase Signaling Pathway to Regulate Lipid Metabolism in Bovine Hepatocytes.


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