Bovine Acetyl Coenzyme A Carboxylase ELISA kit is suitable for the detection of samples from Bovine species. Acetyl Coenzyme A Carboxylase can also be called as ACC.
Specifications Of E11A0913 Bovine ACC ELISA Kit
Product Information | |
Cat. NO. | E11A0913 |
Product Name | Bovine Acetyl Coenzyme A Carboxylase ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
A competitive enzyme immunoassay approach is used in the ACC ELISA kit along with an anti-ACC antibody and an ACC-HRP conjugate. For one hour, the ACC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the ACC concentration since ACC from samples and ACC-HRP conjugate compete for the anti-ACC antibody binding site. Since the number of sites is limited, as more sites are occupied by ACC from the sample, fewer sites are left to bind ACC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACC concentration in each sample is interpolated from this standard curve. |
Quality Control On Bovine ACC ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 92-106 | |
Linearity | Diluent ratio | Range % |
1:2 | 89-109 | |
1:4 | 88-112 | |
1:8 | 85-111 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between ACC and analogues was observed. | |
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Summary Of The Assay Procedures For E11A0913 Bovine ACC ELISA Kit
Citations Of Bovine Acetyl Coenzyme A Carboxylase ELISA Kit
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