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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit (E11C0339)

E11C0339 Bovine C Type Natriuretic Peptide ELISA kit

Bovine C Type Natriuretic Peptide ELISA kit is suitable for the detection of samples from Bovine species. C Type Natriuretic Peptide can also be called NPPC; CNP2; Natriuretic peptide precursor C; natriuretic peptide C, CTNP.

Products

Specifications of Bovine C Type Natriuretic Peptide ELISA kit

Product Information

Cat. No.

E11C0339

Product Name

Bovine C Type Natriuretic Peptide ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CNP antibody and an CNP-HRP conjugate. The assay sample and buffer are incubated together with CNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNP concentration since CNP from samples and CNP-HRP conjugate compete for the anti-CNP antibody binding site. Since the number of sites is limited, as more sites are occupied by CNP from the sample, fewer sites are left to bind CNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNP concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine C Type Natriuretic Peptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

90-101%

Linearity

Diluent Ratio

Range %

1:2

87-105

1:4

84-108

1:8

81-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CNP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine C Type Natriuretic Peptide ELISA kit

Summary of the Assay Procedure for Bovine C Type Natriuretic Peptide ELISA kit

Citations of Bovine C Type Natriuretic Peptide ELISA kit

E11C0339 has been referenced in the below publications:

Can extracellular vesicles from bovine ovarian follicular fluid modulate the in-vitro oocyte meiosis progression similarly to the CNP-NPR2 system?

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