Bovine C Type Natriuretic Peptide ELISA kit is suitable for the detection of samples from Bovine species. C Type Natriuretic Peptide can also be called as NPPC; CNP2; Natriuretic peptide precursor C; natriuretic peptide C.
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Specifications Of E11C0339 Bovine CNP ELISA Kit
Bovine C Type Natriuretic Peptide ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
CNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CNP antibody and an CNP-HRP conjugate. The assay sample and buffer are incubated together with CNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNP concentration since CNP from samples and CNP-HRP conjugate compete for the anti-CNP antibody binding site. Since the number of sites is limited, as more sites are occupied by CNP from the sample, fewer sites are left to bind CNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNP concentration in each sample is interpolated from this standard curve.
Quality Control On Bovine CNP ELISA Kit
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between CNP and analogues was observed.
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Summary Of The Assay Procedures For E11C0339 Bovine CNP ELISA Kit
Citations Of Bovine C Type Natriuretic Peptide ELISA Kit
Can extracellular vesicles from bovine ovarian follicular fluid modulate the in-vitro oocyte meiosis progression similarly to the CNP-NPR2 system?
Product: E11C0339 Bovine C Type Natriuretic Peptide ELISA kit;