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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Luteinizing Hormone ELISA kit

E11L0021 Bovine Luteinizing Hormone ELISA kit

The Bovine Luteinizing Hormone ELISA kit can be used to identify samples from the bovine species. Luteinizing Hormone can also be called ICSH, Lutropin, Interstitial Cell Stimulating Hormone, LH.

Specifications of Bovine Luteinizing Hormone ELISA kit

Product Information

Cat. No.

E11L0021

Product Name

Bovine Luteinizing Hormone ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LH ELISA kit uses an anti-LH antibody and an LH-HRP conjugate in a competitive enzyme immunoassay method. LH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LH antibody binding site between LH from samples and LH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LH. Since the number of sites is limited, as more sites are occupied by LH from the sample, fewer sites are left to bind LH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Luteinizing Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Luteinizing Hormone ELISA kit

Summary of the Assay Procedure for Bovine Luteinizing Hormone ELISA kit

Citations of Bovine Luteinizing Hormone ELISA kit

E11L0021 has been referenced in the below publications:

impact of fertility-promoting powder on trace elements and hormone in serum of dairy cattle with ovarian static disease.

A Study on the Impact of Reproductive Hormones in Serum by Using Fertility-Promoting Powder for Treating Infertility Caused by Persistent Corpus Luteum and Ovary Sandstill of Dairy Cattle.

The Study on the Therapy Options of Uterine Involution in Postpartum Cows.

中药复方理囊散对奶牛子宫复旧期间生殖激素的影响。

The correlation analysis of trace elements and hormones in serum of infertile dairy cattle with ovarian stationary treated with fertility-promoting powder.

Correlation Analysis of IL and Hormone in Serum During Treatment of Persistent Courpus Luteum with Fertility-promoting Power in Dairy Cows.

Rule Analysis of LH,E2 and P4 of Ovulation-Delayed Dairy Cows at Different Stages after Estrus.

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