En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Decorin ELISA kit (E11D0145)

E11D0145 Bovine Decorin ELISA kit

Bovine Decorin ELISA kit is suitable for the detection of samples from Bovine species. Decorin can also be called CSCD; DSPG2; PG40; PGII; PGS2; PG-S2; SLRR1B; Bone proteoglycan II, DCN.

Products

Specifications of Bovine Decorin ELISA kit

Product Information

Cat. No.

E11D0145

Product Name

Bovine Decorin ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

DCN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DCN antibody and an DCN-HRP conjugate. The assay sample and buffer are incubated together with DCN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DCN concentration since DCN from samples and DCN-HRP conjugate compete for the anti-DCN antibody binding site. Since the number of sites is limited, as more sites are occupied by DCN from the sample, fewer sites are left to bind DCN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DCN concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Decorin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

90-103%

Linearity

Diluent Ratio

Range %

1:2

89-105

1:4

86-103

1:8

84-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between DCN and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Decorin ELISA kit

Summary of the Assay Procedure for Bovine Decorin ELISA kit

Citations of Bovine Decorin ELISA kit

E11H0033 has been referenced in the below publications:

Contribution of cross-links and proteoglycans in intramuscular connective tissue to shear force in bovine muscle with different marbling levels and maturities.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.