En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Goat Interleukin 6 ELISA kit

E06I0006 Goat Interleukin 6 ELISA kit

Goat Interleukin 6 ELISA kit is suitable for the detection of samples from goat species. Interleukin 6 can also be called Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating factor, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL6 protein, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin 6, IL-6, IL 6, IL6.

Specifications of Goat Interleukin 6 ELISA kit

Product Information

Cat. No.

E06I0006

Product Name

Goat Interleukin 6 ELISA kit

Species

Goat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 6 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 6 antibody and a IL 6-HRP conjugate. The assay sample and buffer are incubated together with IL 6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 6 concentration since IL 6 from samples and IL 6-HRP conjugate compete for the anti- IL 6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 6 from the sample, fewer sites are left to bind IL 6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 6 concentration in each sample is interpolated from this standard curve.


Quality Control on Goat Interleukin 6 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-116.86%

Linearity

Diluent Ratio

Range %

1:2

92-111

1:4

88-106

1:8

85-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 6 and analogues was observed.



BlueGene Biotech Product Show

Summary of the Assay Procedure for Goat Interleukin 6 ELISA kit

Summary of the Assay Procedure for Goat Interleukin 6 ELISA kit

Citations of Goat Interleukin 6 ELISA kit

E06I0006 has been referenced in the below publications:

The effects of acute lipopolysaccharide challenge on dairy goat liver metabolism assessed with 1HNMR metabonomics.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.