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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Goat Nuclear factor kB ELISA kit

E06N0011 Goat Nuclear factor kB ELISA kit

Goat Nuclear factor kB ELISA kit is suitable for the detection of samples from Goat species. Nuclear factor kB can also be called NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit, NFkB.

Specifications of Goat Nuclear factor kB ELISA kit

Product Information

Cat. NO.

E06N0011

Product Name

Goat Nuclear factor kB ELISA kit

Species

Goat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

A competitive enzyme immunoassay approach is used in the NFkB ELISA kit along with an anti-NFkB antibody and an NFkB-HRP conjugate. For one hour, the NFkB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the NFkB concentration since NFkB from samples and NFkB-HRP conjugate compete for the anti-NFkB antibody binding site. Since the number of sites is limited, as more sites are occupied by NFkB from the sample, fewer sites are left to bind NFkB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFkB concentration in each sample is interpolated from this standard curve.


Quality Control on Goat Nuclear factor kB ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-106%

Linearity

Diluent Ratio

Range %

1:2

89-109

1:4

88-112

1:8

85-111

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NFkB and analogues was observed.


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Summary of the Assay Procedure for Goat Nuclear factor kB ELISA kit

Summary of the Assay Procedure for Goat Nuclear factor kB ELISA kit

Citations of Goat Nuclear factor kB ELISA kit

E06N0011 has been referenced in the below publications:

Study on the mechanism of KangJing Decoction On animal model Cervical Intervertebral Disc Degeneration of animal model.

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