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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Guinea Pig Leukotriene B4 ELISA kit

E05L0037 Guinea Pig Leukotriene B4 ELISA kit

Guinea Pig Leukotriene B4 ELISA kit is suitable for the detection of samples from Guinea Pig species. Leukotriene B4 can also be called LTB4, LT-B4.

Specifications of Guinea Pig Leukotriene B4 ELISA kit

Product Information

Cat. NO.

E05L0037

Product Name

Guinea Pig Leukotriene B4 ELISA kit

Species

Guinea Pig

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LTB4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LTB4 antibody and an LTB4-HRP conjugate. The assay sample and buffer are incubated together with LTB4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LTB4 antibody binding site between LTB4 from samples and LTB4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LTB4. As more sites are taken up by LTB4 from the sample, fewer sites are left that can bind LTB4-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the LTB4 concentration in each sample.


Quality Control on Guinea Pig Leukotriene B4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-106%

Linearity

Diluent Ratio

Range %

1:2

93-103

1:4

89-108

1:8

86-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LTB4 and analogues was observed.


BlueGene Biotech Product

Summary of the Assay Procedure for Guinea Pig Leukotriene B4 ELISA kit

Summary of the Assay Procedure for Guinea Pig Leukotriene B4 ELISA kit

Citations of Guinea Pig Leukotriene B4 ELISA kit

E05L0037 has been referenced in the below publications:

Effects of Eliminating Wind and Dispersing Lung Formula on HIS, LTB4 and NE in plasma of the guinea pigs with airway hypersensitivity.

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