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BlueGene Biotech Human 8 Isoprostaglandin F2a ELISA kit (E01P0207)

E01P0207 Human 8 Isoprostaglandin F2a ELISA kit

The Human 8 Isoprostaglandin F2a ELISA kit can be used to identify samples from the human species. 8 Isoprostaglandin F2a can also be called 8isoPGF2a, 8-iso-PGF2α, 8-Epi-prostaglandin F2α, 8-iso Prostaglandin F2β, (5Z,8β,9α,11α,13E,15S)-9,11,15-Trihydroxyprosta-5,13-dien-1-oic acid1.

Products

Specifications of Human 8 Isoprostaglandin F2a ELISA kit

Product Information

Cat. No.

E01P0207

Product Name

Human 8 Isoprostaglandin F2a ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

8isoPGF2a ELISA kit uses an anti-8isoPGF2a antibody and an 8isoPGF2a-HRP conjugate in a competitive enzyme immunoassay method. 8isoPGF2a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-8isoPGF2a antibody binding site between 8isoPGF2a from samples and 8isoPGF2a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 8isoPGF2a. Since the number of sites is limited, as more sites are occupied by 8isoPGF2a from the sample, fewer sites are left to bind 8isoPGF2a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 8isoPGF2a concentration in each sample is interpolated from this standard curve.


Quality Control on Human 8 Isoprostaglandin F2a ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 8isoPGF2a and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human 8 Isoprostaglandin F2a ELISA kit

Summary of the Assay Procedure for Human 8 Isoprostaglandin F2a ELISA kit

Citations of Human 8 Isoprostaglandin F2a ELISA kit

E01P0207 has been referenced in the below publications:

Hydrogen-rich water decreases serum low-density lipoprotein cholesterol levels and improves high-density lipoprotein function in patients with potential metabolic syndrome.

The evaluation and clinical research of high resolution computed tomography (HRCT) in patients with chronic obstructive pulmonary disease phenotypic.

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