Human Chemokine C C motif ligand 18 ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Chemokine C C motif ligand 18 ELISA kit

E01C0064 Human Chemokine C C motif ligand 18 ELISA kit

The Human Chemokine C C motif ligand 18 ELISA kit can be used to identify samples from the human species. Chemokine C C motif ligand 18 can also be called CCCL18, CCL18, chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated), AMAC-1, AMAC1, CKb7, DC-CK1, DCCK1, MIP-4, PARC, SCYA18, C-C motif chemokine ligand 18, C-C motif chemokine 18, Alternative macrophage activation-associated CC chemokine 1, CC chemokine PARC, Dendritic cell chemokine 1, Macrophage inflammatory protein 4, Pulmonary and activation-regulated chemokine, Small-inducible cytokine A18.

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Specifications of Human Chemokine C C motif ligand 18 ELISA kit

Product Information
Cat. No.	E01C0064
Product Name	Human Chemokine C C motif ligand 18 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	250-5000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	250 pg/mL	1 vial
STANDARD C (0.5mL)	500 pg/mL	1 vial
STANDARD D (0.5mL)	1000 pg/mL	1 vial
STANDARD E (0.5mL)	2500 pg/mL	1 vial
STANDARD F (0.5mL)	5000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CCCL18 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CCCL18. Standards or samples are then added to the microtiter plate wells and CCCL18 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CCCL18 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CCCL18 are added to each well to “sandwich” the CCCL18 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound coCCCL18nents. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CCCL18 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCCL18 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Chemokine C C motif ligand 18 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CCCL18 and analogues was observed.