E01S0013 Human Second mitochondrial activator of caspase ELISA kit
The Human Second mitochondrial activator of caspase ELISA kit can be used to identify samples from the bovine species. Second mitochondrial activator of caspase can also be called Smac, DIABLO.
Product Information | |
Cat. No. | E01S0013 |
Product Name | Human Second mitochondrial activator of caspase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Smac/DIABLO ELISA kit uses an anti-Smac/DIABLO antibody and an Smac/DIABLO-HRP conjugate in a competitive enzyme immunoassay method. Smac/DIABLO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Smac/DIABLO antibody binding site between Smac/DIABLO from samples and Smac/DIABLO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Smac/DIABLO. Since the number of sites is limited, as more sites are occupied by Smac/DIABLO from the sample, fewer sites are left to bind Smac/DIABLO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Smac/DIABLO concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Smac/DIABLO and analogues was observed. | |
E01S0013 has been referenced in the below publications：
Chronic atrophic gastritis Precancerous lesion of gastric cancer turbidity toxin intrinsic card with Livin Smac/DIABLO and Caspase-3 correlation studies.
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