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BlueGene Biotech Human Soluble CD36 ELISA kit (E01S0230)

E01S0230 Human Soluble CD36 ELISA kit

The Human Soluble CD36 ELISA kit can be used to identify samples from the human species. Soluble CD36 can also be called sBDPLT10, sCHDS7, sFAT, sGP3B, sGP4,sGPIV, sPASIV, sSCARB3, sCD36 molecule.

Products

Specifications of Human Soluble CD36 ELISA kit

Product Information

Cat. No.

E01S0230

Product Name

Human Soluble CD36 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

sCD36 ELISA kit uses an anti-sCD36 antibody and an sCD36-HRP conjugate in a competitive enzyme immunoassay method. sCD36-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sCD36 antibody binding site between sCD36 from samples and sCD36-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sCD36. Since the number of sites is limited, as more sites are occupied by sCD36 from the sample, fewer sites are left to bind sCD36-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD36 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Soluble CD36 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between sCD36 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Soluble CD36 ELISA kit

Summary of the Assay Procedure for Human Soluble CD36 ELISA kit

Citations of Human Soluble CD36 ELISA kit

E01S0230 has been referenced in the below publications:

Plasma soluble CD36, carotid intima-media thickness and cognitive function in patients with type 2 diabetes.

Frequency of CD36 deficiency in Thais analyzed by quantification of CD36 on cell surfaces and in plasma.

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