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BlueGene Biotech Human Total Homocystein ELISA kit

E01H0031 Human Total Homocystein ELISA kit

The Human Total Homocystein ELISA kit can be used to identify samples from the human species. Total Homocystein can also be called Homocysteine, Hcy.

Specifications of Human Total Homocystein ELISA kit

Product Information

Cat. No.

E01H0031

Product Name

Human Total Homocystein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 µmoL/L

Sensitivity

1.0 µmoL/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 µmoL/L

1 vial

STANDARD B (0.5mL)

50 µmoL/L

1 vial

STANDARD C (0.5mL)

100 µmoL/L

1 vial

STANDARD D (0.5mL)

250 µmoL/L

1 vial

STANDARD E (0.5mL)

500 µmoL/L

1 vial

STANDARD F (0.5mL)

1000 µmoL/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HCY ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HCY antibody and an HCY-HRP conjugate. The assay sample and buffer are incubated together with HCY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HCY concentration since HCY from samples and HCY-HRP conjugate compete for the anti-HCY antibody binding site. Since the number of sites is limited, as more sites are occupied by HCY from the sample, fewer sites are left to bind HCY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HCY concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Total Homocystein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HCY and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Total Homocystein ELISA kit

Summary of the Assay Procedure for Human Total Homocystein ELISA kit

Citations of Human Total Homocystein ELISA kit

E01H0031 has been referenced in the below publications:

ASSOCIATION OF THROMBOMODULIN GENE METHYLATION WITH THE ONSET OF CEREBRAL INFARCTION.

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