E01U0008 Human Undercarboxylated Osteocalcin ELISA kit
The Human Undercarboxylated Osteocalcin ELISA kit can be used to identify samples from the Human species. Undercarboxylated Osteocalcin can also be called ucOC.
Specifications of Human Undercarboxylated Osteocalcin ELISA kit
Product Information | |
Cat. No. | E01U0008 |
Product Name | Human Undercarboxylated Osteocalcin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ucOC ELISA kit uses an anti-ucOC antibody and an ucOC-HRP conjugate in a competitive enzyme immunoassay method. ucOC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ucOC antibody binding site between ucOC from samples and ucOC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ucOC. Since the number of sites is limited, as more sites are occupied by ucOC from the sample, fewer sites are left to bind ucOC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ucOC concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Undercarboxylated Osteocalcin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ucOC and analogues was observed. | |
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Summary of the Assay Procedure for Human Undercarboxylated Osteocalcin ELISA kit
Citations of Human Undercarboxylated Osteocalcin ELISA kit
E01U0008 has been referenced in the below publications：
Short-chain fatty acids regulate systemic bone mass and protect from pathological bone loss.
Statins, vascular calcification, and vitamin K-dependent proteins: Is there a relation?
