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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Amylase Alpha  2, Pancreatic  ELISA kit (E03A0475)

E03A0475 Mouse Amylase Alpha  2, Pancreatic  ELISA kit

The Mouse Amylase Alpha  2, Pancreatic  ELISA kit can be used to identify samples from the mouse species. Amylase Alpha  2, Pancreatic can also be called AMY2.

Products

Specifications of Mouse Amylase Alpha  2, Pancreatic  ELISA kit

Product Information

Cat. No.

E03A0475

Product Name

Mouse Amylase Alpha  2, Pancreatic  ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

2.5 ng/ml

1 vial

STANDARD C (0.5mL)

5.0 ng/ml

1 vial

STANDARD D (0.5mL)

10 ng/ml

1 vial

STANDARD E (0.5mL)

25 ng/ml

1 vial

STANDARD F (0.5mL)

50 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AMY2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMY2 antibody and an AMY2-HRP conjugate. The assay sample and buffer are incubated together with AMY2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMY2 concentration since AMY2 from samples and AMY2-HRP conjugate compete for the anti-AMY2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AMY2 from the sample, fewer sites are left to bind AMY2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMY2 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Amylase Alpha  2, Pancreatic  ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AMY2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Amylase Alpha  2, Pancreatic  ELISA kit

Summary of the Assay Procedure for Mouse Amylase Alpha  2, Pancreatic  ELISA kit

Citations of Mouse Amylase Alpha  2, Pancreatic  ELISA kit

E03A0475 has been referenced in the below publications:

The use of urinary and kidney SILAM proteomics to monitor kidney response to high dose morpholino oligonucleotides in the mdx mouse.


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