E03A0475 Mouse Amylase Alpha 2, Pancreatic ELISA kit
The Mouse Amylase Alpha 2, Pancreatic ELISA kit can be used to identify samples from the mouse species. Amylase Alpha 2, Pancreatic can also be called AMY2.
Specifications of Mouse Amylase Alpha 2, Pancreatic ELISA kit
Product Information | |
Cat. No. | E03A0475 |
Product Name | Mouse Amylase Alpha 2, Pancreatic ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 10 ng/ml | 1 vial |
STANDARD E (0.5mL) | 25 ng/ml | 1 vial |
STANDARD F (0.5mL) | 50 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AMY2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMY2 antibody and an AMY2-HRP conjugate. The assay sample and buffer are incubated together with AMY2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMY2 concentration since AMY2 from samples and AMY2-HRP conjugate compete for the anti-AMY2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AMY2 from the sample, fewer sites are left to bind AMY2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMY2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Amylase Alpha 2, Pancreatic ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AMY2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Amylase Alpha 2, Pancreatic ELISA kit
Citations of Mouse Amylase Alpha 2, Pancreatic ELISA kit
E03A0475 has been referenced in the below publications：
The use of urinary and kidney SILAM proteomics to monitor kidney response to high dose morpholino oligonucleotides in the mdx mouse.
