E03D0033 Mouse Deoxypyridinoline ELISA kit
The Mouse Deoxypyridinoline ELISA kit can be used to identify samples from the mouse species. Deoxypyridinoline can also be called DPD, DPYD, DHPDHase, Dihydrothymine dehydrogenase, and Dihydrouracil dehydrogenase.
Product Information | |
Cat. No. | E03D0033 |
Product Name | Mouse Deoxypyridinoline ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500nmol/L |
Sensitivity | 1.0nmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 nmol/L | 1 vial |
STANDARD B (0.5mL) | 25 nmol/L | 1 vial |
STANDARD C (0.5mL) | 50 nmol/L | 1 vial |
STANDARD D (0.5mL) | 100 nmol/L | 1 vial |
STANDARD E (0.5mL) | 250 nmol/L | 1 vial |
STANDARD F (0.5mL) | 500 nmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
DPD ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DPD antibody and an DPD-HRP conjugate. The assay sample and buffer are incubated together with DPD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DPD concentration since DPD from samples and DPD-HRP conjugate compete for the anti-DPD antibody binding site. Since the number of sites is limited, as more sites are occupied by DPD from the sample, fewer sites are left to bind DPD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DPD concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between DPD and analogues was observed. | |
E03D0033 has been referenced in the below publications：
Metabolism and mechanism of articular chondrocytes in young Wistar rats exposed to deoxynivalenol.
Contribution of Inflammation and Bone Destruction to Pain in Arthritis: A Study in Murine Glucose-6-Phosphate Isomerase-Induced Arthritis.
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