En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Free Fatty Acid ELISA kit

E03F0002 Mouse Free Fatty Acid ELISA kit

The Mouse Free Fatty Acid ELISA kit can be used to identify samples from the mouse species. Free Fatty Acid can also be called NEFA, nonestesterified fatty acid, free fatty acid, or FFA.

Specifications of Mouse Free Fatty Acid ELISA kit

Product Information

Cat. No.

E03F0002

Product Name

Mouse Free Fatty Acid ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000ug/ml

Sensitivity

1.0ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/ml1 vial

STANDARD B (0.5mL)

50 ug/ml

1 vial

STANDARD C (0.5mL)

100 ug/ml

1 vial

STANDARD D (0.5mL)

250 ug/ml

1 vial

STANDARD E (0.5mL)

500 ug/ml

1 vial

STANDARD F (0.5mL)

1000 ug/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FFA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FFA antibody and an FFA-HRP conjugate. The assay sample and buffer are incubated together with FFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FFA concentration since FFA from samples and FFA-HRP conjugate compete for the anti-FFA antibody binding site. Since the number of sites is limited, as more sites are occupied by FFA from the sample, fewer sites are left to bind FFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FFA concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Free Fatty Acid ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FFA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Free Fatty Acid ELISA kit

Summary of the Assay Procedure for Mouse Free Fatty Acid ELISA kit

Citations of Mouse Free Fatty Acid ELISA kit

E03F0002 has been referenced in the below publications:

Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.

Western diet induces colonic nitrergic myenteric neuropathy and dysmotility in mice via saturated fatty acid- and lipopolysaccharide-induced TLR4 signalling.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.