E03K0027 Mouse Klotho ELISA kit
The Mouse Klotho ELISA kit can be used to identify samples from the mouse species. Klotho can also be called KLa, Klotho Alpha, KL.
Specifications of Mouse Klotho ELISA kit
Product Information | |
Cat. No. | E03K0027 |
Product Name | Mouse Klotho ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
KL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-KL antibody and an KL-HRP conjugate. The assay sample and buffer are incubated together with KL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KL concentration since KL from samples and KL-HRP conjugate compete for the anti-KL antibody binding site. Since the number of sites is limited, as more sites are occupied by KL from the sample, fewer sites are left to bind KL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KL concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Klotho ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between KL and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Klotho ELISA kit
Citations of Mouse Klotho ELISA kit
E03K0027 has been referenced in the below publications：
Tetrahydroxystilbene glucoside extends mouse life span via upregulating neural klotho and downregulating neural insulin or insulin-like growth factor 1.
