E03M0347 Mouse Motilin ELISA kit
The Mouse Motilin ELISA kit can be used to identify samples from the mouse species. Motilin can also be called Motilin, MOT, MLN, Motilin-associated peptide, Promotilin, MTL.
Specifications of Mouse Motilin ELISA kit
Product Information | |
Cat. No. | E03M0347 |
Product Name | Mouse Motilin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000poml/L |
Sensitivity | 1.0poml/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 poml/L | 1 vial |
STANDARD B (0.5mL) | 50 poml/L | 1 vial |
STANDARD C (0.5mL) | 100 poml/L | 1 vial |
STANDARD D (0.5mL) | 250 poml/L | 1 vial |
STANDARD E (0.5mL) | 500 poml/L | 1 vial |
STANDARD F (0.5mL) | 1000 poml/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MTL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MTL antibody and an MTL-HRP conjugate. The assay sample and buffer are incubated together with MTL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTL concentration since MTL from samples and MTL-HRP conjugate compete for the anti-MTL antibody binding site. Since the number of sites is limited, as more sites are occupied by MTL from the sample, fewer sites are left to bind MTL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTL concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Motilin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MTL and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Motilin ELISA kit
Citations of Mouse Motilin ELISA kit
E03M0347 has been referenced in the below publications：
Changes in Small Intestinal Motility and Related Hormones by Acupuncture Stimulation at Zusanli (ST 36) in Mice.
