E03O0341 Mouse Oxidized Lowdensity Lipoprotein ELISA kit
The Mouse Oxidized Lowdensity Lipoprotein ELISA kit can be used to identify samples from the mouse species. Oxidized Lowdensity Lipoprotein can also be called OL, OxLDL.
Specifications of Mouse Oxidized Lowdensity Lipoprotein ELISA kit
Product Information | |
Cat. No. | E03O0341 |
Product Name | Mouse Oxidized Lowdensity Lipoprotein ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100ug/ml |
Sensitivity | 1.0ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 10 ug/mL | 1 vial |
STANDARD D (0.5mL) | 25 ug/mL | 1 vial |
STANDARD E (0.5mL) | 50 ug/mL | 1 vial |
STANDARD F (0.5mL) | 100 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OxLDL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-OxLDL antibody and an OxLDL-HRP conjugate. The assay sample and buffer are incubated together with OxLDL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OxLDL concentration since OxLDL from samples and OxLDL-HRP conjugate compete for the anti-OxLDL antibody binding site. Since the number of sites is limited, as more sites are occupied by OxLDL from the sample, fewer sites are left to bind OxLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OxLDL concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Oxidized Lowdensity Lipoprotein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OxLDL and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Oxidized Lowdensity Lipoprotein ELISA kit
Citations of Mouse Oxidized Lowdensity Lipoprotein ELISA kit
E03O0341 has been referenced in the below publications：
Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy.
Role of CXCL16 in mice with adriamycin induced nephropathy and the protective effects of simvastatin.
Molecular hydrogen stabilizes atherosclerotic plaque in low-density lipoprotein receptor-knockout mice.
