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BlueGene Biotech Mouse Cytochrome C Oxidase Subunit II ELISA kit (E03C0016)

E03C0016 Mouse Cytochrome C Oxidase Subunit II ELISA kit

The Mouse Cytochrome C Oxidase Subunit II ELISA kit can be used to identify samples from the mouse species. Cytochrome C Oxidase Subunit II can also be called COX2, MTCO2, MT-CO2, COII, COXII, Mitochondrially Encoded Cytochrome C Oxidase II, Cytochrome c oxidase polypeptide II.

Products

Specifications of Mouse Cytochrome C Oxidase Subunit II ELISA kit

Product Information

Cat. No.

E03C0016

Product Name

Mouse Cytochrome C Oxidase Subunit II ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

COX2 ELISA kit uses an anti-COX2 antibody and an COX2-HRP conjugate in a competitive enzyme immunoassay method. COX2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-COX2 antibody binding site between COX2 from samples and COX2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of COX2. Since the number of sites is limited, as more sites are occupied by COX2 from the sample, fewer sites are left to bind COX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX2 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Cytochrome C Oxidase Subunit II ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between COX2 and analogues was observed.


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Summary of the Assay Procedure for Mouse Cytochrome C Oxidase Subunit II ELISA kit

Summary of the Assay Procedure for Mouse Cytochrome C Oxidase Subunit II ELISA kit

Citations of Mouse Cytochrome C Oxidase Subunit II ELISA kit

E03C0016 has been referenced in the below publications:

Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and inhibition of transcription factor activation.

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