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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Protein Kinase A ELISA kit

E03P0746 Mouse Protein Kinase A ELISA kit

The Mouse Protein Kinase A ELISA kit can be used to identify samples from the mouse species. Protein Kinase A can also be called cAMP-dependent protein kinase, PKA.

Specifications of Mouse Protein Kinase A ELISA kit

Product Information

Cat. No.

E03P0746

Product Name

Mouse Protein Kinase A ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PKA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PKA antibody and a PKA-HRP conjugate. The assay sample and buffer are incubated together with PKA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKA concentration since PKA from samples and PKA-HRP conjugate compete for the anti-PKA antibody binding site. Since the number of sites is limited, as more sites are occupied by PKA from the sample, fewer sites are left to bind PKA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKA concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Protein Kinase A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PKA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Protein Kinase A ELISA kit

Summary of the Assay Procedure for Mouse Protein Kinase A ELISA kit

Citations of Mouse Protein Kinase A ELISA kit

E03P0746 has been referenced in the below publications:

Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.

Effect of medium chain triglycerides on brown adipose tissue and its β3 adrenergic receptors in mice with obesity induced by high fat diet.

Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.

Effect of medium chain triglycerides on brown adipose tissue in obese mice.

Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.

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