E03P0578 Mouse Procollagen Type Ⅰ N Terminal Propeptide ELISA kit
The Mouse Procollagen Type Ⅰ N Terminal Propeptide ELISA kit can be used to identify samples from the mouse species. Procollagen Type Ⅰ N Terminal Propeptide can also be called P1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide.
Product Information | |
Cat. No. | E03P0578 |
Product Name | Mouse Procollagen Type Ⅰ N Terminal Propeptide ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PⅠNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PⅠNP antibody and an PⅠNP-HRP conjugate. The assay sample and buffer are incubated together with PⅠNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PⅠNP concentration since PⅠNP from samples and PⅠNP-HRP conjugate compete for the anti-PⅠNP antibody binding site. Since the number of sites is limited, as more sites are occupied by PⅠNP from the sample, fewer sites are left to bind PⅠNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PⅠNP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PⅠNP and analogues was observed. | |
E03P0578 has been referenced in the below publications：
Inhibition of microRNA‑34a ameliorates murine collagen‑induced arthritis.
Naringin ameliorates bone loss induced by sciatic neurectomy and increases Semaphorin 3A expression in denervated bone.
Osteoglycin silencing exerts inhibitory effects on myocardial fibrosis and epithelial/endothelial-mesenchymal transformation in a mouse model of myocarditis.
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