E03S0014 Mouse Serum Albumin ELISA kit
The Mouse Serum Albumin ELISA kit can be used to identify samples from the mouse species. Serum Albumin can also be called SALB.
Specifications of Mouse Serum Albumin ELISA kit
Product Information | |
Cat. No. | E03S0014 |
Product Name | Mouse Serum Albumin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000ng/mL |
Sensitivity | 1.0ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SALB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SALB antibody and a SALB-HRP conjugate. The assay sample and buffer are incubated together with SALB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SALB concentration since SALB from samples and SALB-HRP conjugate compete for the anti-SALB antibody binding site. Since the number of sites is limited, as more sites are occupied by SALB from the sample, fewer sites are left to bind SALB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SALB concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Serum Albumin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SALB and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Serum Albumin ELISA kit
Citations of Mouse Serum Albumin ELISA kit
E03S0014 has been referenced in the below publications：
Treatment of α-1 antitrypsin deficiency using hepatic-specified cells derived from human-induced pluripotent stem cells.
