E03S0266 Mouse Soluble Leptin Receptor ELISA kit
The Mouse Soluble Leptin Receptor ELISA kit can be used to identify samples from the mouse species. Soluble Leptin Receptor can also be called sLeptin R, sLeptin receptor, sCD297, sLEP-R, sLR, sLEPRD, sOB-R, sOBR.
Product Information | |
Cat. No. | E03S0266 |
Product Name | Mouse Soluble Leptin Receptor ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SLR ELISA kit uses an anti-SLR antibody and an SLR-HRP conjugate in a competitive enzyme immunoassay method. SLR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SLR antibody binding site between SLR from samples and SLR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SLR. Since the number of sites is limited, as more sites are occupied by SLR from the sample, fewer sites are left to bind SLR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLR concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SLR and analogues was observed. | |
E03S0266 has been referenced in the below publications：
CB1R regulates soluble leptin receptor levels via CHOP, contributing to hepatic leptin resistance.
Resveratrol intake during pregnancy and lactation re-programs adiposity and ameliorates leptin resistance in male progeny induced by maternal high-fat/high sucrose plus postnatal high-fat/highsucrose diets via fat metabolism regulation.
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