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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Cardiac Troponin T ELISA kit

E02T0017 Rat Cardiac Troponin T ELISA kit

The Rat Cardiac Troponin T ELISA kit can be used to identify samples from the rat species. Cardiac Troponin T can also be called cTnT, Cardiac muscle troponin T, CMH2, TnTc, CardiomyopathyHypertrophic 2, CMD1D, CMPD2.

Specifications of Rat Cardiac Troponin T ELISA kit

Product Information

Cat. No.

E02T0017

Product Name

Rat Cardiac Troponin T ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

cTnT ELISA kit uses an anti-cTnT antibody and an cTnT-HRP conjugate in a competitive enzyme immunoassay method. cTnT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-cTnT antibody binding site between cTnT from samples and cTnT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of cTnT. Since the number of sites is limited, as more sites are occupied by cTnT from the sample, fewer sites are left to bind cTnT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cTnT concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Cardiac Troponin T ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between cTnT and analogues was observed.


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Summary of the Assay Procedure for Rat Cardiac Troponin T ELISA kit

Summary of the Assay Procedure for Rat Cardiac Troponin T ELISA kit

Citations of Rat Cardiac Troponin T ELISA kit

E02T0017 has been referenced in the below publications:

Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation..

细胞代数与5-氮胞苷浓度对体外诱导骨髓间充质干细胞向心肌样细胞定向分化的影响。

Effect of propofol on autophagy during ischemia-reperfusion injury to rat hearts.


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