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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Endothelin 1 ELISA kit (E02E0040)

E02E0040 Rat Endothelin 1 ELISA kit

The Rat Endothelin 1 ELISA kit can be used to identify samples from the rat species. Creatine Kinase can also be called Endothelin-1, Big endothelin 1, EDN1 protein, Endothelin1, ET1,  PPET1, Preproendothelin 1.

Products

Specifications of Rat Endothelin 1 ELISA kit

Product Information

Cat. No.

E02E0040

Product Name

Rat Endothelin 1 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

EDN1 ELISA kit uses an anti-EDN1 antibody and an EDN1-HRP conjugate in a competitive enzyme immunoassay method. EDN1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-EDN1 antibody binding site between EDN1 from samples and EDN1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of EDN1. Since the number of sites is limited, as more sites are occupied by EDN1 from the sample, fewer sites are left to bind EDN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EDN1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Endothelin 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between EDN1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Endothelin 1 ELISA kit

Summary of the Assay Procedure for Rat Endothelin 1 ELISA kit

Citations of Rat Endothelin 1 ELISA kit

E02E0040 has been referenced in the below publications:

Effects of fasudil on pulmonary arterial hypertension with vascular remodeling in rats.

cAMP-PKA-CaMKII signaling pathway is involved in aggravated cardiotoxicity during Fuzi and Beimu Combination Treatment of Experimental Pulmonary Hypertension.

The therapeutic effect and mechanism of betaine and enalapril alone and combined use in the renal hypertensive rats.

Effect of propofol on autophagy during ischemia-reperfusion injury to rat hearts.

Study on Mechanism of Yiqi Huoxue Compound Anti-arterial Thrombosis and Its Action.

THE STUDY OF FASUDIL HYDROCHLORIDE PREVENTED ACUTE KIDNEY INJURY RAT CAUSED BY LIPOPOLYSACCHARIDE.

Effect of adiponectin on hepatic stellate cell contraction induced by endothelin-1 and its mechanism of action.


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