E02E0040 Rat Endothelin 1 ELISA kit
The Rat Endothelin 1 ELISA kit can be used to identify samples from the rat species. Creatine Kinase can also be called Endothelin-1, Big endothelin 1, EDN1 protein, Endothelin1, ET1, PPET1, Preproendothelin 1.
Specifications of Rat Endothelin 1 ELISA kit
Product Information | |
Cat. No. | E02E0040 |
Product Name | Rat Endothelin 1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
EDN1 ELISA kit uses an anti-EDN1 antibody and an EDN1-HRP conjugate in a competitive enzyme immunoassay method. EDN1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-EDN1 antibody binding site between EDN1 from samples and EDN1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of EDN1. Since the number of sites is limited, as more sites are occupied by EDN1 from the sample, fewer sites are left to bind EDN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EDN1 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Endothelin 1 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between EDN1 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Endothelin 1 ELISA kit
Citations of Rat Endothelin 1 ELISA kit
E02E0040 has been referenced in the below publications：
Effects of fasudil on pulmonary arterial hypertension with vascular remodeling in rats.
cAMP-PKA-CaMKII signaling pathway is involved in aggravated cardiotoxicity during Fuzi and Beimu Combination Treatment of Experimental Pulmonary Hypertension.
The therapeutic effect and mechanism of betaine and enalapril alone and combined use in the renal hypertensive rats.
Effect of propofol on autophagy during ischemia-reperfusion injury to rat hearts.
Study on Mechanism of Yiqi Huoxue Compound Anti-arterial Thrombosis and Its Action.
THE STUDY OF FASUDIL HYDROCHLORIDE PREVENTED ACUTE KIDNEY INJURY RAT CAUSED BY LIPOPOLYSACCHARIDE.
Effect of adiponectin on hepatic stellate cell contraction induced by endothelin-1 and its mechanism of action.
