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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Surfactant P ELISA kit

E02S0272 Rat Surfactant P ELISA kit

The Rat Surfactant P ELISA kit can be used to identify samples from the rat species. Surfactant P can also be called SP.

Specifications of Rat Surfactant P ELISA kit

Product Information

Cat. No.

E02S0272

Product Name

Rat Surfactant P ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SP ELISA kit uses an anti-SP antibody and an SP-HRP conjugate in a competitive enzyme immunoassay method. SP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SP antibody binding site between SP from samples and SP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SP. Since the number of sites is limited, as more sites are occupied by SP from the sample, fewer sites are left to bind SP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Surfactant P ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Surfactant P ELISA kit

Summary of the Assay Procedure for Rat Surfactant P ELISA kit

Citations of Rat Surfactant P ELISA kit

E02S0272 has been referenced in the below publications:

Effects of ischemic preconditioning on expression of VEGF, GLUT1 and BAI1 in focal cerebral infarction rats.

Effects and mechanism ofelectro-acupuncture on constipation and diarrhea type of irritable bowel syndrome in rats.

Effects of KuiKeLing and Its Modified Formula on Bowel Movement and Gastrointestinal Peptide Hormones in Colitis Model Rats.

The Liver Injury Mechamisms In Rats With Sever Acute Pancreatitis And Protective Effects Of N-acetylcysteine Homocysteine To Liver.

Downregulated microRNA-92a-3p inhibits apoptosis and promotes proliferation of pancreatic acinar cells in acute pancreatitis by enhancing KLF2 expression.

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