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  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Superoxide Dismutase ELISA kit (E14S0012)

E14S0012 Sheep Superoxide Dismutase ELISA kit

Sheep Superoxide Dismutase ELISA kit is suitable for the detection of samples from sheep species. Superoxide Dismutase can also be called SOD1, Cu-Zn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble, ALS, ALS1, amyotrophic lateral sclerosis 1, adult, Cu, Zn superoxide dismutase, hSod1, indophenoloxidase A, IPOA, SOD, Superoxide dismutase 1, superoxide dismutase 1, soluble, superoxide dismutase, cystolic.

Products

Specifications of Sheep Superoxide Dismutase ELISA kit

Product Information

Cat. No.

E14S0012

Product Name

Sheep Superoxide Dismutase ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SOD ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SOD antibody and an SOD-HRP conjugate. The assay sample and buffer are incubated together with SOD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOD concentration since SOD from samples and SOD-HRP conjugate compete for the anti-SOD antibody binding site. Since the number of sites is limited, as more sites are occupied by SOD from the sample, fewer sites are left to bind SOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Superoxide Dismutase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SOD and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Superoxide Dismutase ELISA kit

Summary of the Assay Procedure for Sheep Superoxide Dismutase ELISA kit

Citations of Sheep Superoxide Dismutase ELISA kit

E14S0012 has been referenced in the below publications:

Effects of α-Lipoic Acid on Growth Performance,Antioxidant and Immunity of Sheep under Heat Stress.

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