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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Glutathione peroxidase ELISA kit (E11G0369)

E11G0369 Bovine Glutathione peroxidase ELISA kit

The Bovine Glutathione peroxidase ELISA kit can be used to identify samples from the bovine species. Glutathione peroxidase can also be called GPx, GSHPx.

Products

Specifications of Bovine Glutathione peroxidase ELISA kit

Product Information

Cat. No.

E11G0369

Product Name

Bovine Glutathione peroxidase ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GSH Px ELISA kit uses an anti-GSH Px antibody and an GSH Px-HRP conjugate in a competitive enzyme immunoassay method. GSH Px-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GSH Px antibody binding site between GSH Px from samples and GSH Px-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GSH Px. Since the number of sites is limited, as more sites are occupied by GSH Px from the sample, fewer sites are left to bind GSH Px-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSH Px concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Glutathione peroxidase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GSH Px and analogues was observed.


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Summary of the Assay Procedure for Bovine Glutathione peroxidase ELISA kit

Summary of the Assay Procedure for Bovine Glutathione peroxidase ELISA kit

Citations of Bovine Glutathione peroxidase ELISA kit

E11G0369 has been referenced in the below publications:

Low Expression of Sirtuin 1 in the Dairy Bovines with Mild Fatty Liver Alters Hepatic Lipid Metabolism.

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