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  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit (E12G0016)

E12G0016 Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit is suitable for the detection of samples from Chicken species. Granulocyte Macrophage Colony Stimulating Factor can also be called CSF2, GMCSF,  colony stimulating factor 2, CSF, GM CSF.

Products

Specifications of Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Product Information

Cat. No.

E12G0016

Product Name

Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GM CSF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GM CSF antibody and an GM CSF-HRP conjugate. The assay sample and buffer are incubated together with GM CSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GM CSF concentration since GM CSF from samples and GM CSF-HRP conjugate compete for the anti-GM CSF antibody binding site. Since the number of sites is limited, as more sites are occupied by GM CSF from the sample, fewer sites are left to bind GM CSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GM CSF concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GM CSF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Summary of the Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Citations of Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit

E12G0016 has been referenced in the below publications:

Effects of Chinese Herbal Compound Polysaccharides on Immune Functions during the Brood Incubation Period in Different MHC B-Lβ II Genotype of Laying Hens.

Effects of compound Chinese herbal medicin al polysaccharides on Immunomodulatory in different MHC B-LBⅡ Genotype Egg-laying Chicks.


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