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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Adrenocorticotropic Hormone ELISA kit

E02A0005 Rat Adrenocorticotropic Hormone ELISA kit

The Rat Adrenocorticotropic Hormone ELISA kit is suitable for the detection of samples from Rat species. Adrenocorticotropic Hormone can also be called OMC, Pro-opiomelanocortin, Corticotropin-lipotropin, Adrenocorticotropic, ACTH.

Specifications of Rat Adrenocorticotropic Hormone ELISA kit

Product Information

Cat. No.

E02A0005

Product Name

Rat Adrenocorticotropic Hormone ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial



Principle of the Assay

ACTH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ACTH antibody and an ACTH-HRP conjugate. The assay sample and buffer are incubated together with ACTH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTH concentration since ACTH from samples and ACTH-HRP conjugate compete for the anti-ACTH antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTH from the sample, fewer sites are left to bind ACTH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTH concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Adrenocorticotropic Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-109%

Linearity

Diluent Ratio

Range %

1:2

92-101

1:4

91-107

1:8

90-113

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ACTH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Adrenocorticotropic Hormone ELISA kit

Summary of the Assay Procedure for Rat Adrenocorticotropic Hormone ELISA kit

Citations of Rat Adrenocorticotropic Hormone ELISA kit

E02A0005 has been referenced in the below publications:

Transcutaneous Auricular Vagus Nerve Stimulation at 20 Hz Improves Depression-Like Behaviors and Down-Regulates the Hyperactivity of HPA Axis in Chronic Unpredictable Mild Stress Model Rats.

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