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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Ferritin ELISA kit

E08F0010 Canine Ferritin ELISA kit

Canine Ferritin ELISA kit is suitable for the detection of samples from Canine species. Ferritin can also be called FE.

Specifications of Canine Ferritin ELISA kit

Product Information

Cat. No.

E08F0010

Product Name

Canine Ferritin ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FE ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FE antibody and an FE-HRP conjugate. The assay sample and buffer are incubated together with FE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FE concentration since FE from samples and FE-HRP conjugate compete for the anti-FE antibody binding site. Since the number of sites is limited, as more sites are occupied by FE from the sample, fewer sites are left to bind FE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FE concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Ferritin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-108%

Linearity

Diluent Ratio

Range %

1:2

87-113

1:4

82-116

1:8

84-115

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FE and analogues was observed.



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Summary of the Assay Procedure for Canine Ferritin ELISA kit

Summary of the Assay Procedure for Canine Ferritin ELISA kit

Citations of Canine Ferritin ELISA kit

E08F0010 has been referenced in the below publications:

Analysis of blood degradation products and ferritin in the cerebrospinal fluid of dogs with acute thoracolumbar intervertebral disk extrusion, a prospective pilot study.

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