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BlueGene Biotech Human Apolipoprotein C3 ELISA kit

E01A0516 Human Apolipoprotein C3 ELISA kit

The Human Apolipoprotein C3 ELISA kit can be used to identify samples from the human species. Apolipoprotein C3 can also be called APOC3, ApoC III, ApoCIII, Apolipoprotein C III, Apolipoprotein C3.

Specifications of Human Apolipoprotein C3 ELISA kit

Product Information

Cat. No.

E01A0516

Product Name

Human Apolipoprotein C3 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

250 ng/mL

1 vial

STANDARD C (0.5mL)

500 ng/mL

1 vial

STANDARD D (0.5mL)

1000 ng/mL

1 vial

STANDARD E (0.5mL)

2500 ng/mL

1 vial

STANDARD F (0.5mL)

5000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

APOC3 ELISA kit uses an anti-APOC3 antibody and an APOC3-HRP conjugate in a competitive enzyme immunoassay method. APOC3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-APOC3 antibody binding site between APOC3 from samples and APOC3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of APOC3. Since the number of sites is limited, as more sites are occupied by APOC3 from the sample, fewer sites are left to bind APOC3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOC3 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Apolipoprotein C3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between APOC3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Apolipoprotein C3 ELISA kit

Summary of the Assay Procedure for Human Apolipoprotein C3 ELISA kit

Citations of Human Apolipoprotein C3 ELISA kit

E01A0516 has been referenced in the below publications:

Lack of association between apolipoprotein C3 gene polymorphisms and risk of nonalcoholic fatty liver disease in a Chinese Han population.

Association between APOC3 promoter region polymorphisms and non-alcoholic fatty liver disease.

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