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  • bovine serum albumin elisa 1

BlueGene Biotech Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

E01P0001 Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

The Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit can be used to identify samples from the human species. ATP-Binding Cassette, Sub-Family B, Member 1 can also be called ABCB1, ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, ATP binding cassette subfamily B member 1, P-glycoprotein, P-glycoprotein 1, permeability glycoprotein, P-gp, Pgp, multidrug resistance protein 1, MDR1, ATP-binding cassette sub-family B member 1, ABCB1, cluster of differentiation 243.

Specifications of Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

Product Information

Cat. No.

E01P0001

Product Name

Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/ml

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ABCB1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ABCB1. Standards or samples are then added to the microtiter plate wells and ABCB1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ABCB1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ABCB1 are added to each well to “sandwich” the ABCB1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ABCB1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCB1 concentration in each sample is interpolated from this standard curve. 


Quality Control on Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ABCB1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

Summary of the Assay Procedure for Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

Citations of Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

E01P0001 has been referenced in the below publications:

Correlations of ABCB1 gene C3435T polymorphism with serum and cerebrospinal fluid P-gp expression in Han people with epilepsy.

Relationship of P-glycoprotein expression of serum and cerebrospinal fluid and epilepsy drug-resistance in Hui and Han People with epilepsy.

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