E01C0096 Human Caspase 9 ELISA kit
The Human Caspase 9 ELISA kit can be used to identify samples from the human species. Caspase 9 can also be called CASP9, MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6.
Human Caspase 9 ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
CASP9 ELISA kit uses an anti-CASP9 antibody and an CASP9-HRP conjugate in a competitive enzyme immunoassay method. CASP9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CASP9 antibody binding site between CASP9 from samples and CASP9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CASP9. Since the number of sites is limited, as more sites are occupied by CASP9 from the sample, fewer sites are left to bind CASP9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP9 concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between CASP9 and analogues was observed.
E01C0096 has been referenced in the below publications：
Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning.
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