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BlueGene Biotech Human Caspase 9 ELISA kit (E01C0096)

E01C0096 Human Caspase 9 ELISA kit

The Human Caspase 9 ELISA kit can be used to identify samples from the human species. Caspase 9 can also be called CASP9, MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6.

Products

Specifications of Human Caspase 9 ELISA kit

Product Information

Cat. No.

E01C0096

Product Name

Human Caspase 9 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CASP9 ELISA kit uses an anti-CASP9 antibody and an CASP9-HRP conjugate in a competitive enzyme immunoassay method. CASP9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CASP9 antibody binding site between CASP9 from samples and CASP9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CASP9. Since the number of sites is limited, as more sites are occupied by CASP9 from the sample, fewer sites are left to bind CASP9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP9 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Caspase 9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CASP9 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Caspase 9 ELISA kit

Summary of the Assay Procedure for Human Caspase 9 ELISA kit

Citations of Human Caspase 9 ELISA kit

E01C0096 has been referenced in the below publications:

Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning.

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