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  • bovine serum albumin elisa 1

BlueGene Biotech Human Triosephosphate isomerase ELISA kit

E01T0727 Human Triosephosphate isomerase ELISA kit

The Human Triosephosphate isomerase ELISA kit can be used to identify samples from the human species. Triosephosphate isomerase can also be called phosphotriose isomerase, triose phosphoisomerase, triose phosphate mutase, D-glyceraldehyde-3-phosphate ketol-isomerase.

Specifications of Human Triosephosphate isomerase ELISA kit

Product Information

Cat. No.

E01T0727

Product Name

Human Triosephosphate isomerase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TPI1 ELISA kit uses an anti-TPI1 antibody and an TPI1-HRP conjugate in a competitive enzyme immunoassay method. TPI1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TPI1 antibody binding site between TPI1 from samples and TPI1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TPI1. Since the number of sites is limited, as more sites are occupied by TPI1 from the sample, fewer sites are left to bind TPI1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TPI1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Triosephosphate isomerase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TPI1 and analogues was observed.


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Summary of the Assay Procedure for Human Triosephosphate isomerase ELISA kit

Summary of the Assay Procedure for Human Triosephosphate isomerase ELISA kit

Citations of Human Triosephosphate isomerase ELISA kit

E01T0727 has been referenced in the below publications:

cAMP-PKA-CaMKII signaling pathway is involved in aggravated cardiotoxicity during Fuzi and Beimu Combination Treatment of Experimental Pulmonary Hypertension.

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