E03C0042 Mouse C Peptide ELISA kit
The Mouse C Peptide ELISA kit can be used to identify samples from the mouse species. C Peptide can also be called C P.
Mouse C Peptide ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
C P ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-C P antibody and a C P-HRP conjugate. The assay sample and buffer are incubated together with C P-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C P concentration since C P from samples and C P-HRP conjugate compete for the anti-C P antibody binding site. Since the number of sites is limited, as more sites are occupied by C P from the sample, fewer sites are left to bind C P-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C P concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between C P and analogues was observed.
E03C0042 has been referenced in the below publications：
Establishment and preliminary evaluation of alloxan diabetic models with three mouse strains.
Related Bluegene Biotech Products