E03G0264 Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit
The Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit can be used to identify samples from the mouse species. Glycogen Synthase Kinase 3 Alpha can also be called GSK3a, GSK3A, Gsk3a, glycogen synthase kinase 3 alpha, Serine/threonine-protein kinase GSK3A.
Product Information | |
Cat. No. | E03G0264 |
Product Name | Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GSK3a ELISA kit uses an anti-GSK3a antibody and an GSK3a-HRP conjugate in a competitive enzyme immunoassay method. GSK3a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GSK3a antibody binding site between GSK3a from samples and GSK3a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GSK3a. Since the number of sites is limited, as more sites are occupied by GSK3a from the sample, fewer sites are left to bind GSK3a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSK3a concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GSK3a and analogues was observed. | |
E03G0264 has been referenced in the below publications：
Effect of Different Factors of Moxibustion on P13K／AKT Signal Pathway and Cortical 6-amyioid Protein Precipitation in Brain Tissue of Alzheimer Mice.
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