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BlueGene Biotech Mouse Mast Cell Tryptase ELISA kit (E03M0256)

E03M0256 Mouse Mast Cell Tryptase ELISA kit 

The Mouse Mast Cell Tryptase ELISA kit  can be used to identify samples from the mouse species. Mast Cell Tryptase can also be called Tryptase delta, Delta-tryptase, HmMCP-3-like tryptase III, Mast cell mMCP-7-like, Tryptase-3, TPSD1, MCP7L1, MMCP-7L, MCP7-LIKE, EC 3.4.21.59, MGC95428, TRYD, Tryptase3, alpha II, EC 3.4.21.59, Lung tryptase, Mast cell alpha II tryptase, Mast cell beta I tryptase, Mast cell protease 7, Mast cell protease II, MCP 7, MMCP 7, Pituitary tryptase, Skin tryptase, TPS 1, TPS1, TPS2, TPSAB1, TPSAB1 protein, TPSB1, Tryptase 1, Tryptase alpha 1, tryptase alpha I included, Tryptase alpha II, MCT.

Products

Specifications of Mouse Mast Cell Tryptase ELISA kit

Product Information

Cat. No.

E03M0256

Product Name

Mouse Mast Cell Tryptase ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

100-2500pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MCT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MCT antibody and an MCT-HRP conjugate. The assay sample and buffer are incubated together with MCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCT concentration since MCT from samples and MCT-HRP conjugate compete for the anti-MCT antibody binding site. Since the number of sites is limited, as more sites are occupied by MCT from the sample, fewer sites are left to bind MCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCT concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Mast Cell Tryptase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MCT and analogues was observed.


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Summary of the Assay Procedure for Mouse Mast Cell Tryptase ELISA kit

Summary of the Assay Procedure for Mouse Mast Cell Tryptase ELISA kit

Citations of Mouse Mast Cell Tryptase ELISA kit

E03M0256 has been referenced in the below publications:

Electroacupuncture in conscious free-moving mice reduces pain by ameliorating peripheral and central nociceptive mechanisms.

 


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