Mouse Mast Cell Tryptase ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Mast Cell Tryptase ELISA kit

E03M0256 Mouse Mast Cell Tryptase ELISA kit

The Mouse Mast Cell Tryptase ELISA kit can be used to identify samples from the mouse species. Mast Cell Tryptase can also be called Tryptase delta, Delta-tryptase, HmMCP-3-like tryptase III, Mast cell mMCP-7-like, Tryptase-3, TPSD1, MCP7L1, MMCP-7L, MCP7-LIKE, EC 3.4.21.59, MGC95428, TRYD, Tryptase3, alpha II, EC 3.4.21.59, Lung tryptase, Mast cell alpha II tryptase, Mast cell beta I tryptase, Mast cell protease 7, Mast cell protease II, MCP 7, MMCP 7, Pituitary tryptase, Skin tryptase, TPS 1, TPS1, TPS2, TPSAB1, TPSAB1 protein, TPSB1, Tryptase 1, Tryptase alpha 1, tryptase alpha I included, Tryptase alpha II, MCT.

Products

Specifications of Mouse Mast Cell Tryptase ELISA kit

Product Information
Cat. No.	E03M0256
Product Name	Mouse Mast Cell Tryptase ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	100-2500pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	100 pg/mL	1 vial
STANDARD C (0.5mL)	250 pg/mL	1 vial
STANDARD D (0.5mL)	500 pg/mL	1 vial
STANDARD E (0.5mL)	1000 pg/mL	1 vial
STANDARD F (0.5mL)	2500 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MCT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MCT antibody and an MCT-HRP conjugate. The assay sample and buffer are incubated together with MCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCT concentration since MCT from samples and MCT-HRP conjugate compete for the anti-MCT antibody binding site. Since the number of sites is limited, as more sites are occupied by MCT from the sample, fewer sites are left to bind MCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCT concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Mast Cell Tryptase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MCT and analogues was observed.