E03M0040 Mouse Methylglyoxal ELISA kit
The Mouse Methylglyoxal ELISA kit can be used to identify samples from the mouse species. Methylglyoxal can also be called Pyruvaldehyde, MG.
Specifications of Mouse Methylglyoxal ELISA kit
Product Information | |
Cat. No. | E03M0040 |
Product Name | Mouse Methylglyoxal ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 10 ng/ml | 1 vial |
STANDARD D (0.5mL) | 25 ng/ml | 1 vial |
STANDARD E (0.5mL) | 50 ng/ml | 1 vial |
STANDARD F (0.5mL) | 100 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MG antibody and an MG-HRP conjugate. The assay sample and buffer are incubated together with MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MG concentration since MG from samples and MG-HRP conjugate compete for the anti-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by MG from the sample, fewer sites are left to bind MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MG concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Methylglyoxal ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MG and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Methylglyoxal ELISA kit
Citations of Mouse Methylglyoxal ELISA kit
E03M0040 has been referenced in the below publications：
Metformin prevents methylglyoxal-induced apoptosis by suppressing oxidative stress in vitro and in vivo.
