E03G0367 Mouse Reduced Glutathione ELISA kit
The Mouse Reduced Glutathione ELISA kit can be used to identify samples from the mouse species. Reduced Glutathione can also be called L-Reduced Glutathione, GSH.
Specifications of Mouse Reduced Glutathione ELISA kit
Product Information | |
Cat. No. | E03G0367 |
Product Name | Mouse Reduced Glutathione ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GSH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GSH antibody and an GSH-HRP conjugate. The assay sample and buffer are incubated together with GSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSH concentration since GSH from samples and GSH-HRP conjugate compete for the anti-GSH antibody binding site. Since the number of sites is limited, as more sites are occupied by GSH from the sample, fewer sites are left to bind GSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSH concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Reduced Glutathione ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GSH and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Reduced Glutathione ELISA kit
Citations of Mouse Reduced Glutathione ELISA kit
E03G0367 has been referenced in the below publications：
Protective effects of apigenin and myricetin against cisplatin-induced nephrotoxicity in mice.
