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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Bcl2 Associated Death Promoter ELISA kit

E02B0633 Rat Bcl2 Associated Death Promoter ELISA kit

The Rat Bcl2 Associated Death Promoter ELISA kit can be used to identify samples from the rat species. Bcl2 Associated Death Promoter can also be called  BAD, BBC2, BCL2L8, BCL2 associated agonist of cell death.

Specifications of Rat Bcl2 Associated Death Promoter ELISA kit

Product Information

Cat. No.

E02B0633

Product Name

Rat Bcl2 Associated Death Promoter ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BAD ELISA kit uses an anti-BAD antibody and an BAD-HRP conjugate in a competitive enzyme immunoassay method. BAD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BAD antibody binding site between BAD from samples and BAD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BAD. Since the number of sites is limited, as more sites are occupied by BAD from the sample, fewer sites are left to bind BAD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAD concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Bcl2 Associated Death Promoter ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BAD and analogues was observed.


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Summary of the Assay Procedure for Rat Bcl2 Associated Death Promoter ELISA kit

Summary of the Assay Procedure for Rat Bcl2 Associated Death Promoter ELISA kit

Citations of Rat Bcl2 Associated Death Promoter ELISA kit

E02B0633 has been referenced in the below publications:

Identification of Chronic Atrophic Gastritis Model Rats Differentially Expressed Proteins in Gastric Mucosa by Quantitative Proteomics and Research for Effect Targets of Chinese Medicine.


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