E02P0028 Rat Plasminogen Activator Inhibitor 1 ELISA kit
The Rat Plasminogen Activator Inhibitor 1 ELISA kit can be used to identify samples from the rat species. Plasminogen Activator Inhibitor 1 can also be called SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor.
Product Information | |
Cat. No. | E02P0028 |
Product Name | Rat Plasminogen Activator Inhibitor 1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PAI 1 ELISA kit uses an anti-PAI 1 antibody and an PAI 1-HRP conjugate in a competitive enzyme immunoassay method. PAI 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PAI 1 antibody binding site between PAI 1 from samples and PAI 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PAI 1. Since the number of sites is limited, as more sites are occupied by PAI 1 from the sample, fewer sites are left to bind PAI 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAI 1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PAI 1 and analogues was observed. | |
E02P0028 has been referenced in the below publications：
Effect of different compatibility of huayu-tongluo Chinese herbs on the kidney of diabetic nephropathy rats the expression of integrin alpha 3 beta 1 and the related proteins in wnt/beta-catenin pathway.
Study on Hemostatic Effect and Mechanism of Polysaccharides from Bletilla striata in Blood Heat and Hemorrhage Model Rats.
Influence of Endothelial Function and Blood Ｒheology in Diabetic Ｒats by Paeonol and Tanshinol.
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