Rat S100 Calcium Binding Protein B ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat S100 Calcium Binding Protein B ELISA kit

Epigenetics and Nuclear Signaling

Cell Biology

Stem Cells

E02S0042 Rat S100 Calcium Binding Protein B ELISA kit

The Rat S100 Calcium Binding Protein B ELISA kit can be used to identify samples from the rat species. S100 Calcium Binding Protein B can also be called NEF, Protein S100 B, Protein S100-B, S 100 calcium binding protein beta chain, S 100 protein beta chain, S 100 protein beta subunit, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 calcium binding protein B, S100 calcium binding protein beta (neural), S100 calcium binding protein beta chain, S100 calcium-binding protein B, S100 protein beta chain, S100beta, S100B.

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Specifications of Rat S100 Calcium Binding Protein B ELISA kit

Product Information
Cat. No.	E02S0042
Product Name	Rat S100 Calcium Binding Protein B ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

S100B ELISA kit uses an anti-S100B antibody and an S100B-HRP conjugate in a competitive enzyme immunoassay method. S100B-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-S100B antibody binding site between S100B from samples and S100B-HRP conjugate, the intensity of the color is inversely proportional to the concentration of S100B. Since the number of sites is limited, as more sites are occupied by S100B from the sample, fewer sites are left to bind S100B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100B concentration in each sample is interpolated from this standard curve.

Quality Control on Rat S100 Calcium Binding Protein B ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between S100B and analogues was observed.

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Summary of the Assay Procedure for Rat S100 Calcium Binding Protein B ELISA kit

Citations of Rat S100 Calcium Binding Protein B ELISA kit

E02S0042 has been referenced in the below publications：

Effects of Starvation Following by Refeeding on Cerebral Injury in Rats.

Limited fluid resuscitation inhibits S100B protein expression in the cerebral tissue of rats with hemorrhagic shock.

Effects of Early Acupuncture on Serum GFAP and S-100B Protein in Rats with Traumatic Brain Injury.

Protective effect of naloxone injected into cisterna magna on brain tissues of rats following cardiopulmonary resuscitation.

Influence of different resuscitation strategies on SIOOB and receptor of advanced glycation end—products(RAGE)in rat brain tissue after hemorrhagic shock.

Protective Effect of Mild hypothermia on Cerebral Injury after Cardiac Arrest Cardiopulmonary Resuscitation in Anoxia induced Rats.