E02S0006 Rat Stromal cell Derived factor 1Alpha ELISA kit
The Rat Stromal cell Derived factor 1Alpha ELISA kit can be used to identify samples from the rat species. Stromal cell Derived factor 1Alpha can also be called SDF-1-alpha, SDF-1a, CXCL12a, SDF1A, SDF1Alpha.
Product Information | |
Cat. No. | E02S0006 |
Product Name | Rat Stromal cell Derived factor 1Alpha ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SDF1Alpha ELISA kit uses an anti-SDF1Alpha antibody and an SDF1Alpha-HRP conjugate in a competitive enzyme immunoassay method. SDF1Alpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SDF1Alpha antibody binding site between SDF1Alpha from samples and SDF1Alpha-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SDF1Alpha. Since the number of sites is limited, as more sites are occupied by SDF1Alpha from the sample, fewer sites are left to bind SDF1Alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF1Alpha concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SDF1Alpha and analogues was observed. | |
E02S0006 has been referenced in the below publications：
The application of collagen-binding SDF and functional collagen scaffoldsfor spinal cord injury repair and myocardial regeneration.
Nano-Silicate-Reinforced and SDF-1α-Loaded Gelatin-Methacryloyl Hydrogel for Bone Tissue Engineering.
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