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BlueGene Biotech Rat Stromal cell Derived factor 1Alpha ELISA kit

E02S0006 Rat Stromal cell Derived factor 1Alpha ELISA kit

The Rat Stromal cell Derived factor 1Alpha ELISA kit can be used to identify samples from the rat species. Stromal cell Derived factor 1Alpha can also be called SDF-1-alpha, SDF-1a, CXCL12a, SDF1A, SDF1Alpha.

Specifications of Rat Stromal cell Derived factor 1Alpha ELISA kit

Product Information

Cat. No.

E02S0006

Product Name

Rat Stromal cell Derived factor 1Alpha ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SDF1Alpha ELISA kit uses an anti-SDF1Alpha antibody and an SDF1Alpha-HRP conjugate in a competitive enzyme immunoassay method. SDF1Alpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SDF1Alpha antibody binding site between SDF1Alpha from samples and SDF1Alpha-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SDF1Alpha. Since the number of sites is limited, as more sites are occupied by SDF1Alpha from the sample, fewer sites are left to bind SDF1Alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF1Alpha concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Stromal cell Derived factor 1Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SDF1Alpha and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Stromal cell Derived factor 1Alpha ELISA kit

Summary of the Assay Procedure for Rat Stromal cell Derived factor 1Alpha ELISA kit

Citations of Rat Stromal cell Derived factor 1Alpha ELISA kit

E02S0006 has been referenced in the below publications:

The application of collagen-binding SDF and functional collagen scaffoldsfor spinal cord injury repair and myocardial regeneration.

Nano-Silicate-Reinforced and SDF-1α-Loaded Gelatin-Methacryloyl Hydrogel for Bone Tissue Engineering.

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