E14C2509 Sheep Calcium Binding Protein D9K ELISA kit
Sheep Calcium Binding Protein D9K ELISA kit is suitable for the detection of samples from sheep species. Calcium Binding Protein D9K can also be called CABP1, Calb3, Cabp9k, AU042539, CaBP-D9K, CABPD9k.
Product Information | |
Cat. No. | E14C2509 |
Product Name | Sheep Calcium Binding Protein D9K ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CABPD9k ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CABPD9k antibody and an CABPD9k-HRP conjugate. The assay sample and buffer are incubated together with CABPD9k-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CABPD9k concentration since CABPD9k from samples and CABPD9k-HRP conjugate compete for the anti-CABPD9k antibody binding site. Since the number of sites is limited, as more sites are occupied by CABPD9k from the sample, fewer sites are left to bind CABPD9k-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CABPD9k concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CABPD9k and analogues was observed. | |
E14C2509 has been referenced in the below publications:
Synergy Effects of Low Dietary Cation-Anion Diffe rence with Vitamin D on Plasma Concentrations of Calcium and Its Modulators of Pregnant Goats.
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