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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Sirtuin 1 ELISA kit

E11S0444 Bovine Sirtuin 1 ELISA kit

The Bovine Sirtuin 1 ELISA kit can be used to identify samples from the bovine species. Sirtuin 1 can also be called SIR2L1, Silent Mating Type Information Regulation 2 Homolog 1, NAD-Dependent Deacetylase Sirtuin-1, SIR2-like protein 1, SirtT1 75 kDa fragment, SIRT1.

Specifications of Bovine Sirtuin 1 ELISA kit

Product Information

Cat. No.

E11S0444

Product Name

Bovine Sirtuin 1 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SIRT1 ELISA kit uses an anti-SIRT1 antibody and an SIRT1-HRP conjugate in a competitive enzyme immunoassay method. SIRT1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SIRT1 antibody binding site between SIRT1 from samples and SIRT1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SIRT1. Since the number of sites is limited, as more sites are occupied by SIRT1 from the sample, fewer sites are left to bind SIRT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIRT1 concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Sirtuin 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SIRT1 and analogues was observed.


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Summary of the Assay Procedure for Bovine Sirtuin 1 ELISA kit

Summary of the Assay Procedure for Bovine Sirtuin 1 ELISA kit

Citations of Bovine Sirtuin 1 ELISA kit

E11S0444 has been referenced in the below publications:

Low Expression of Sirtuin 1 in the Dairy Bovines with Mild Fatty Liver Alters Hepatic Lipid Metabolism.

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