E01G0201 Human Glucose Transporter 4 ELISA kit
The Human Glucose Transporter 4 ELISA kit can be used to identify samples from the human species. Glucose Transporter 4 can also be called GLUT4, SLC2A4, solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4.
Product Information | |
Cat. No. | E01G0201 |
Product Name | Human Glucose Transporter 4 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GLUT4 ELISA kit uses an anti-GLUT4 antibody and an GLUT4-HRP conjugate in a competitive enzyme immunoassay method. GLUT4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GLUT4 antibody binding site between GLUT4 from samples and GLUT4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GLUT4. Since the number of sites is limited, as more sites are occupied by GLUT4 from the sample, fewer sites are left to bind GLUT4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT4 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GLUT4 and analogues was observed. | |
E01G0201 has been referenced in the below publications：
Experimental studies of the combination effects of berberine and puerarin on NASH in vitro.
A cell model of nonalcoholic steatohepatitis induced by FFA: establishment and dynamic monitoring.
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