E03S0144 Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit
The Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit can be used to identify samples from the mouse species. Superoxide Dismutase 2, Mitochondrial can also be called SOD2, IPOB, MNSOD, MVCD6, IPO-B, Mn-SOD, superoxide dismutase 2, mitochondrial, superoxide dismutase 2, GClnc1.
Product Information | |
Cat. No. | E03S0144 |
Product Name | Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10μg/ml |
Sensitivity | 0.1μg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD F (0.5mL) | 10 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SOD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SOD2 antibody and an SOD2-HRP conjugate. The assay sample and buffer are incubated together with SOD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOD2 concentration since SOD2 from samples and SOD2-HRP conjugate compete for the anti-SOD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SOD2 from the sample, fewer sites are left to bind SOD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SOD2 and analogues was observed. | |
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