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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine cyclic Adenosine Monophosphate ELISA kit

E07C0027 Porcine cyclic Adenosine Monophosphate ELISA kit

The Porcine cyclic Adenosine Monophosphate ELISA kit can be used to identify samples from the porcine species. cyclic Adenosine Monophosphate can also be called cAMP, cyclic AMP, or 3',5'-cyclic adenosine monophosphate, Adenosine 3′,5′-(hydrogen phosphate).

Specifications of Porcine cyclic Adenosine Monophosphate ELISA kit

Product Information

Cat. No.

E07C0027 

Product Name

Porcine cyclic Adenosine Monophosphate ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pmol/mL

Sensitivity

1.0 pmol/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pmol/mL

1 vial

STANDARD B (0.5mL)

50 pmol/mL

1 vial

STANDARD C (0.5mL)

100 pmol/mL

1 vial

STANDARD D (0.5mL)

250 pmol/mL

1 vial

STANDARD E (0.5mL)

500 pmol/mL

1 vial

STANDARD F (0.5mL)

1000 pmol/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

cAMP ELISA kit uses an anti-cAMP antibody and an cAMP-HRP conjugate in a competitive enzyme immunoassay method. cAMP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-cAMP antibody binding site between cAMP from samples and cAMP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of cAMP. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine cyclic Adenosine Monophosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between cAMP and analogues was observed.


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Summary of the Assay Procedure for Porcine cyclic Adenosine Monophosphate ELISA kit

Summary of the Assay Procedure for Porcine cyclic Adenosine Monophosphate ELISA kit

Citations of Porcine cyclic Adenosine Monophosphate ELISA kit

E07C0027 has been referenced in the below publications:

An essential role for the intra- oocyte MAPK activity in the NSN- to-SN transition of germinal vesicle chromatin configuration in porcine oocytes.

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